The critically acclaimed laboratory standard for more than fifty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. This new volume presents methods related to the use of bacterial genetics for genomic engineering. The book includes sections on strain collections and genetic nomenclature; transposons; and phage.(If TEV protease expression is IPTG dependent, do not use BactoTryptone in the medium because it contains lactose. Change BactoTryptone ... Lyse cells (e.g., by passing twice through a French press [20, 000 psi]). 6. Purify TEV protease by anbsp;...
|Title||:||Advanced Bacterial Genetics: Use of Transposons and Phage for Genomic Engineering|
|Publisher||:||Academic Press - 2007-02-27|