Caffeic acid phenethyl ester (CAPE), a plant derived polyphenolic compound, has been shown to protect organs from I/R induced damage in vivo, and this effect has been attributed to its antioxidant activity. To better understand the mechanism of CAPE protection, a model using menadione-induced oxidative stress in human endothelial cells to simulate I/R injury in vitro was developed. Gene expression analysis was performed with microarrays undergoing cytoprotection with CAPE. The dose-dependent cytoprotection of CAPE has been related to its induction of heme oxygenase-1 (HO-1).5.2.2 Instrumentation HPLC was performed on a Varian HPLC system equipped with a 9010 pump, a 9050 UV detector, a 9065 diode array detector, and a 9100 autosampler fitted with a 20I¼l sample injection loop. Instruments were controlled anbsp;...
|Title||:||Amelioration of Oxidative Stress in Human Endothelial Cells by Caffeic Acid Phenethyl Ester (CAPE) and Fluorinated Derivatives (FCAPES) and Pharmacokinetic Characterization of CAPE and FCAPE in Rats|
|Publisher||:||ProQuest - 2007|