Animal Cell Electroporation and Electrofusion Protocols

Animal Cell Electroporation and Electrofusion Protocols

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This superb collection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells also includes proven animal cell electrofusion techniques for studies of somatic cell genetics and development, and for generating monoclonal antibodies. It is distinguished by its coverage of important model cell types from many organisms and tissue types and its detailed instructions for the growth and preparation of specific cells to achieve optimum transfection. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible results even for beginners.General circuit diagram of an exponential decay wave form generator. Q, 2, 3 are tne energy storage capacitors, ... RT1, R^, and RT3 are timing resistors, which can be added to the circuit by switches S5]6, 7. If the output voltage is measured atanbsp;...

Title:Animal Cell Electroporation and Electrofusion Protocols
Author:Jac A. Nickoloff
Publisher:Springer Science & Business Media - 1995


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