Antibody Engineering Protocols

Antibody Engineering Protocols

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This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents. Antibody Engineering Protocols constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step ensure that the vector cloning sites are not found in the VH and VL antibody sequences. ... of expressed sFv from the periplasmic space is achieved by osmotic shock followed by immobilized metal affinity chromatography or IMAC ( 18).

Title:Antibody Engineering Protocols
Author:Sudhir Paul
Publisher:Springer Science & Business Media - 1995


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