The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.Usually, the desired gene can then be precisely adapted to this blunt end to form an in-frame fusion by ... In designing the in-frame fusion junction, researchers should note that enterokinase is able to cleave --DDDDKX--, where X can be any amino acid residue except proline. ... 25 J. Sambrook, E. F. Fritsch, and T. Maniatis, in a#39;a#39;Molecular Cloning: A Laboratory Manual, a#39;a#39; 2nd Ed. Cold Spring Harboranbsp;...
|Title||:||Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification|
|Publisher||:||Academic Press - 2000-10-11|