Findings and Conclusions. The pipetting validity of the BiomekRTM 2000 was verified through a series of dye studies. The use of the P20 pipetting tool was found to be unreliable for pipetting 1microL volumes. At increased pipetting volumes, the P20 was not significantly different than an analyst using a certified pipette. The P200 pipetting tool also had no significant difference in pipetting volumes from that of a manual setup. The Q-TAT assay was altered such that the amount of template DNA added to the PCR reaction mixture was increased to 5microL. The assay was successfully implemented onto the robotic platform with no significant difference between standard curves generated using manual and robotic means. A mock sample comparison revealed a C.V. of 30.2% between manual and automated pipetting. Suggested improvements for automation include addition of the internal amplification control to the template human DNA as an indicator that DNA was added to the PCR mixture. Other considerations for successfully automating Q-TAT are implementing the necessary quality assurance/quality control practices that apply when dealing with large number of samples, such as organizational sheets and accurate robotic scripts.... and 0.5I¼L GS-500 LIZ size standard (Applied Biosystems, Foster City, CA). Products in each sample were then electro-injected for 4 seconds onto an ABI 310 Genetic Analyzer for a run time of 19 minutes. For manual samples, the 48- tubeanbsp;...
|Title||:||Automating a Human DNA Quantitation Technique Using a BiomekRTM 2000 Robotic Platform|
|Publisher||:||ProQuest - 2008|