With the growing global fear of a major pandemic, avian influenza (AI) virus research has greatly increased in importance. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental, yet essential, virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. They present exciting, cutting-edge new methods that focus both on studying the virus itself and on work with avian hosts, an area greatly lacking in research.Concentrated stock solutions should be stored at a70 AdC. Primer stock solutions should be 200 I¼M (200 pmol/I¼L) (see Note 6); ... is one cycle of 30min at 50 AdC and 15min at 94 AdC. This reverse-transcription step is specific for the Qiagen OneStep RT-PCR Kit, ... Information on setting up and programming a specific real-time PCR instrument can be found in the usera#39;s manual. 2. Place the reaction tubes in a cooling block, taking care not to damage optical surfaces on reaction tubes. 3.
|Title||:||Avian Influenza Virus|
|Publisher||:||Springer Science & Business Media - 2008-02-28|