Experienced researchers describe in step-by-step detail powerful methods for the investigation of bacterial exo- and endotoxins. These state-of-the-art techniques range from purification and detection methods to methods of conformational analysis, and include the use of phage antibody libraries and methods of structural and functional analyses of endotoxins. Each protocol is described by a scientist who has regularly used the method and optimized it to a high level of performance. Extensive notes deal with the difficulties that may arise when using the method and with the method's limitations and possible modification for other purposes. Cutting-edge and geared to producing successful experimental results, Bacterial Toxins: Methods and Protocols provides investigators with a first-rate collection of readily reproducible methods designed to help today's bacterial toxin investigators - both novice and expert - utilize a wide array of powerful research tools.We use 25a50 mL of this culture to inoculate 1 L of LB. The flasks are shaken vigorously for 3a4 h ... The cells are harvested by centrifugation and stored at a20 AdC before lysis in a French Press. 6. The frozen cell pellets are resuspended in 40anbsp;...
|Publisher||:||Springer Science & Business Media - 2000-01-01|