The focus of this work is EphA2, a member of the Eph family of receptor tyrosine kinases that has been implicated in cellular transformation and metastasis. It is a 110 kDa protein found primarily in adult epithelial cells where is mediates cell-cell contacts. In normal cells, EphA2 is found at low concentrations, but with high levels of tyrosine phosphorylation. This situation is reversed in aggressive epithelial tumor cells, here there is an increase in EphA2 protein levels, and a loss of tyrosine phosphorylation. Use of antibodies to cause EphA2 to dimerize on turmor cells has been found to restore normal protein and phosphorylation levels and cause the cells to revert to a normal phenotype. These observations show EphA2 to be a possible target for cancer therapies.... coli BL21 (DE3)codonplusRP cells (Stratagene), which contain extra tRNAs to correct for arginine and proline bacterial codon bias (Table 2.5, Figure 2.5). Calculation of fusion protein characteristics, including theoretical isoelectric points, was undertaken and suggested that ... translocated via SecB, the major chaperone used by E. coli for exporting protein out of the cell (Novagen, pET System Manual).
|Title||:||Biochemical and Biophysical Studies of the Human Ephrin Receptor Protein Tyrosine Kinase, EphA2|
|Publisher||:||ProQuest - 2006|