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This volume and its companion volume 360 introduce a new topic to the Methods in Enzymology series. They will cover, among other topics, imaging, screening, and diagnosis in biological systems. See key features for greater detail. Key Features * Optical instrumentation for imaging, screening and diagnosis in molecules, tissues, and cells * Development and application of optical probes and techniques for imaging and drug screening, protemics, genomics, and cellomics * Applications of biophotonics research to the understanding of mechanisms of cellular reactions and processes, investigating the structure and dynamics of biomolecular systems, screening and drug discovery, and diagnosis and treatment of diseaseAround 16 hr postfertilization (hpf) the neural plate begins to invaginate (NF14; Nieuwkoop and Faber), taking on a cylindrical conformation. Closure of the neural tube in the Xenopus embryo occurs through stage 20 (22 hpf) and coincides with the earliest pioneer axon outgrowth. ... Remove the jelly coat and vitelline membrane surrounding each embryo by using a blunt forceps (Dumont # 5-Standard tip) to pin the jelly coat down and a ... Schematic diagram of neural tube dissection.

Publisher:Academic Press - 2003-03-11


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