Cardiovascular Proteomics: Methods and Protocols, covers many of the aspects of the proteomic approach in the cardiovascular field. This volume takes the reader through the complete process of proteomic analysis, from the obtention of specific heart proteins (troponin I) to the new techniques of identifying risk biomarkers of atherome plaque rupture analyzing the secretome of explanted endartrectomies cultured in vitro or the application of phage display techniques to decipher the molecular diversity of blood vessels.Methods and Protocols Fernando Vivanco. 3. ... Level the gradient master and perform the adequate run (see instruction manual for the equipment). 4. ... 8. Analyze the fractions as explained in Subheading 3.6., steps 7 and 8. 4. Notes 1. The concentration of all sucrose solutions must be controlled using a refractometer.
|Publisher||:||Springer Science & Business Media - 2007|