SDS-PAGE was performed according to the method of Laemmli157 following detailed instructions obtained from ... The stacking gel was 4 .5% acrylamide (4.5 % T, 2.67% Q. The separating gel was 12.5% acrylamide (12.5% T, 2.67% Q. The sample buffer was 0.1 M TRIS-HC1, pH 6.8, 0.25% ... Electrophoresis was carried out at constant current (25 mAmp) for 1.5 h, when the dye front had migrated to theanbsp;...
|Title||:||Chemistry of Inhibition of Bee Venom Phospholipase A(2) by the Marine Natural Products Manoalide and Luffariellolide|
|Author||:||Barbara Mueller Potts|