Current Protocols in Immunology

Current Protocols in Immunology

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One simple way to alleviate this problem is to perform a two-step immobilization of the antibody by precoating the plate with an anti-immunoglobulin reagent that reacts with the monoclonal. In general, plates may first be coated with 50 |.rl of the anti-lg reagents (20 ng/ml), then washed, and then the ... Prolonged refrigerator storage of antibody solutions and repeated freeze-thaw cycles must be avoided.

Title:Current Protocols in Immunology
Publisher: - 1996


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