Increased turnover of plakophilin-3 during TGF-beta treatment was found to be due to calpain activity. Inhibitors of calpains stabilized plakophilin-3 during TGF-beta treatment, and it was found that the plakophilin-3 head domain was sensitive to calpains. However, plakophilin-2 is not degraded by calpains. These studies show that protease activity contributes to desmosome disassembly during TGF-beta induced EMT.Desmoglein-2 containing the proregion is found at the cell membrane. In typical diagrams showing the trafficking of the classical cadherins, the proregion of E- and N-cadherin is shown being removed ... with a non-membrane- permeable biotinylation reagent, sulfo-N-hydroxysuccinimidobiotin, to label cell surface proteins.
|Title||:||Desmosome Disassembly During TGF-beta Induced Epithelial to Mesenchymal Transition|
|Publisher||:||ProQuest - 2008|