In this study, optical reflectance spectroscopy with short source-detector separation is used as a minimally invasive technique to differentiate normal tissues from tumor lesions ex-vivo. Reflectance spectra (400 nm--1000 nm) were acquired with an optical probe from human specimens after radical or partial nephrectomys and radical prostatectomy. The main aim of my thesis was to differentiate between normal and tumor tissues and then extend the application to differentiate between benign and malignant tissues. The study involved 13 human subjects' specimens for prostate measurements and 23 human subjects's specimens for kidney measurements. My study reveals that for kidney tissues, promising parameters were (1) the slope of the reflectance spectrum from 630 nm to 900 nm, (2) correlation coefficients between the normal and tumor (or between benign and malignant) reflectance spectra from 560 nm to 630 nm, (3) and two-wavelength-intensity classifications. For prostate tissue, promising parameters were the slope of the reflectance spectrum from 630 nm to 900 nm. Furthermore, NIR reflectance spectroscopy can be further used with an optical probe having larger source-detector separations to image deep tissues. A qsmartq probe can be designed which can be used intra-operatively to provide real time surgical guidance during laparoscopic surgery.A student t-test is a fundamental statistical process to compare the two means of two groups of data. Normally ... For our data analysis using Pearsona#39;s correlation calculation, the spectral data sets were not normalized: After subtracting the dark anbsp;...
|Title||:||Detection of Positive Cancer Margins Intra-operatively During Nephrectomy and Prostatectomy Using Optical Reflectance Spectroscopy|
|Publisher||:||ProQuest - 2007|