This is a well thought-out, highly practical text covering contemporary ain vitroa techniques for drug absorption studies. Starting at the molecular level of investigation, it continues with cell monolayer models (both primary and cell lines) and culminates with in situ techniques as a final testing format. In addition, chapters on high-throughput assays, in vitro-in vivo correlation, bioinformatics and regulatory issues are covered, giving a comprehensive overview of available models and techniques. Moreover, an appendix consisting of a number of practical protocols is available online, updated as needed, and should prove very helpful to apply the techniques directly to the benchside.In brief, the rats are anesthetized, followed by an injection of 0.2 mL of the test solution into the common carotid artery. ... and 10 mM HEPES, pH 7.4) which contains both the test substrate (e.g., a [3H]-labeled compound, about 10 I¼Ci) and a ... been used to determine the transport of fluorescein across the BRB in the blood-to-vitreous humor direction as well as in ... have eyes whose size is similar to that of human eyes , the anatomy of the rabbit eye is unusual among mammals inanbsp;...
|Title||:||Drug Absorption Studies|
|Author||:||Carsten Ehrhardt, Kwang-Jin Kim|
|Publisher||:||Springer Science & Business Media - 2007-12-22|