Alan V. Smrcka presents a collection of cutting-edge methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. Among the readily reproducible techniques presented are those for the purification of G proteins and effectors enzymes, assays of these purified G proteins and effector enzymes, and for the study of G proteins interactions with effectors in intact cells. Additional methods are provided for assaying G protein coupled receptor structure, function, and localization, and for studying the physiological roles for endogenous G proteins.... 1310a1317. 4. Srinivasa, S. P., Bernstein, L. A., Blumer, K. J., and Linder, M. E. (1998) Plasma membrane localization is required for RGS4 function in Saccharomyces cerevisiae. ... Chem. 274, 38, 260a38, 267. 7. Druey, K. M., Ugar, O., Caron, J. M., Chen, C.-K., Backlund, P. S., and Jones, T. L. Z. (1999) ... (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Plainview, NY.
|Title||:||G Protein Signaling|
|Author||:||Alan V. Smrcka|
|Publisher||:||Springer Science & Business Media - 2004-01|