Genetic Library Construction and Screening

Genetic Library Construction and Screening

4.11 - 1251 ratings - Source

Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.set up a mock experiment which can be used to practice the mechanical skills necessary. ... References Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current protocols in molecular ... Gene 25 :263-269 Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual.

Title:Genetic Library Construction and Screening
Author:R. Curtis Bird, Bruce Smith
Publisher:Springer Science & Business Media - 2012-12-06


You Must CONTINUE and create a free account to access unlimited downloads & streaming