Guide to Protein Purification

Guide to Protein Purification

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Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for protein and enzyme purification * Personal perspectives on enzyme purification by eminent researchers Among the Topics Covered * General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structureThe toluene serves to prevent bacterial growth and to permeabilize the yeast membrane. ... They include the French press, 7 Eaton press, 8 and Gaulin homogenizer.9 Apart from the Gaulin homogenizer, these instruments are generally designed to handle relatively small ... A batch of beads may be used many times with periodic nitric acid cleaning, if it is washed thoroughly with detergent after each use.

Title:Guide to Protein Purification
Author:Murray P. Deutscher
Publisher:Gulf Professional Publishing - 1990


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