Building upon the foundation of basics discussed in the previous edition, the Second Edition provides a more in-depth look at the latest methods and technologies of advanced drug screening, an essential function of drug discovery. With extensively updated content and 21 new chapters, this text examines: quality and efficiency of drug target validation and potential drug compound selection reducing assay costs and improvement in assay technology data quality improvement trends in drug discovery how drug screening can minimize adverse drug effects recent advancements, including: high content screening; protein-protein interactions; high-throughput crystallization; lead optimization; chemoinformatics and microfluidics technologies; target validation by genomics, proteomics, and siRNA; structure-based drug design; automation; and medicinal chemistryWhen ELISA kits are not available commercially, the protein levels in various cell lines can be quantified using the specific antibody against a target using the Li- COR Odyssey system (http://www.licor.com/bio/odyssey). The in-cell western isanbsp;...
|Title||:||Handbook of Drug Screening, Second Edition|
|Author||:||Ramakrishna Seethala, Litao Zhang|
|Publisher||:||CRC Press - 2009-06-24|