This volume introduces a summary of all the techniques used to estimate pH reliably. Emphasis is placed on the techniques that provide the most reliable and detailed data. The role of cell pH is explained with special emphasis on enzymology and membrane transport and bioenergetics. This book was written especially for molecular biologists, biochemists and biophysicists.The pH profiles across the plasma membrane of the yeast S. cerevisiae suspended in water or buffers at pH 5 or 8. ... The best way to avoid these problems is to keep the exposure times of both pictures under all circumstances equal and, if possible, to diminish the brightness of the ... TV Camera A TV camera, if properly handled and equipped with an image intensifier, represents a 5 FIGURE 56.
|Title||:||Intracellular pH and its Measurement|
|Author||:||Arnost Kotyk, Jan Slavik|
|Publisher||:||CRC Press - 1989-04-30|