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This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is full of step-by-step qhow toq methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies. Key Features * Isolation of mitochondria from commonly used cells and tissues * Assays for mitochondrial activities, including respiration, ATP production, permeability, protein import, and interactions with the cytoskeleton * Biochemical and optical methods for studying protein-protein interactions in mitochondria * Approaches to studying mitochondrial replication, transcription, and translation * Transmitochondrial technologies * Methods in microassay data analysis3 A schematic diagram of an imaging system for tracking mitochondrial motility. ... a Zeiss Axioplan II microscope (Carl Zeiss, Oberkochen, Germany) using a Plan- Apochromat 100A—1.4 NA objective lens, and a Orca1 digital camera (C4742-95, anbsp;...

Publisher:Academic Press - 2001-05-23


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