Volume 2.Table 15. Troubleshooting guide (continued) Problem Possible causeaquot; Remedy Non-specific Probe too short background etc. ... Use a less harsh stripping method with nitrocellulose (e.g. Wash for 2 h at 65AdC in 500 ml 5 mM Tris-HCl pH 7.5, ... aquot;Within each category, the most likely causes of the the problem are listed first. 5.
|Title||:||Molecular Biology Labfax: Gene analysis|
|Author||:||Terence A. Brown|
|Publisher||:||Elsevier - 1998|