Nanoparticle cytotoxicity depends on intracellular solubility: Comparison of stabilized copper metal and degradable copper oxide nanoparticles Abstract Metal nanoparticles have distinctly different chemical and physical properties than currently investigated oxides. Since pure metallic nanoparticles are igniting at air, carbon stabilized copper nanoparticles were used as representative material for this class. Using copper as a representative example, we compare the cytotoxicity of copper metal nanoparticles stabilized by a carbon layer to copper oxide nanoparticles using two different cell lines. Keeping the copper exposure dose constant, the two forms of copper showed a distinctly different response. Whilst copper oxide had already been reported to be highly cytotoxic, carbon-coated copper nanoparticles were much less cytotoxic and more tolerated. Measuring the two material's intra- and extracellular solubility in model buffers explained this difference on the basis of altered copper release when supplying copper metal or the corresponding oxide particles to the cells. Control experiments using pure carbon nanoparticles were used to exclude significant surface effects. Reference experiments with ionic copper solutions confirmed a similar response of cultures if exposed to copper oxide nanoparticles or ionic copper. These observations are in line with a Trojan horse-type mechanism and illustrate the dominating influence of physico-chemical parameters on the cytotoxicity of a given metal. KeywordsEngineered nanoparticles; Mass; Dose; Nanotoxicology; Degradation; Safety; Core/shell; CarbonManufacture and use of nanomaterials: current status in the UK and global trends . .... Hsiao, M.C., Wang, H.P., Yang, Y.W., 2001. ... Biotests and biosensors for ecotoxicology of metal oxide nanoparticles: a minireview. ... 102a110. Miller, R.J., Lenihan, H.S., Muller, E.B., Tseng, N., Hanna, S.K., Keller, A.A., 2010. Impacts of metal oxide nanoparticles on ... Guidance Manual for the testing of manufactured nanomaterials: OECDa#39;s sponsorship programme, ENV/JM/MONO(2009) 20REV.
|Title||:||Nanoparticle cytotoxicity depends on intracellular solubility: Comparison of stabilized copper metal and degradable copper oxide nanoparticles|
|Author||:||Andreas M. Studer , Ludwig K. Limbach , Luu Van Duc , Frank Krumeich , Evagelos K. Athanassiou , Lukas C. Gerber , Holger Moch , Wendelin J. Stark|
|Publisher||:||Science World Library -|