Chlorosomes are the light-harvesting antennae of the green sulfur bacteria, which usually live in extremely light-limited environments. In Chlorobium tepidum, ten kinds of proteins (CsmA, CsmB, CsmC, CsmD, CsmE, CsmF, CsmH, CsmI, CsmJ, and CsmX) are located on the chlorosome envelope membrane. Cross-linking experiments were performed to detect the relative locations and interactions among the chlorosome envelope proteins. CsmA forms homomultimers in the baseplate and interacts with the Fenna-Matthews-Olson protein. The pre-CsmA, CsmB and CsmF proteins interact with CsmA on the edge of the CsmA baseplate. CsmC forms homomultimers and it might be located on the opposite side from the baseplate facing the cytoplasm. Iron-sulfur chlorosome proteins CsmI and CsmJ form heterodimers, and both interact with CsmB. A model of the protein organization of the chlorosome membrane is proposed based on the cross-linking information.The C. tepidum cells were harvested from a 2-L culture by centrifugation and were disrupted by three passages through a chilled French press (124 MPa, 4 oC ) ... The purified chlorosomes were aliquoted and stored at -80 oC for future use.
|Title||:||Organization and Function of Chlorosome Proteins in the Green Sulfur Bacterium Chlorobium Tepidum|
|Publisher||:||ProQuest - 2006|