Proteins interacting with diverse ligands - proteins, peptides or DNA - are the basic principles underlying many biological processes, such as antigen-antibody binding, signal transduction or receptor binding. The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.The SPOT method was originally developed as a manual method and steps common to all spot reactors are carried out manually by washing the whole ... ASP 222 automated spot robot was developed to reliably deliver amino acid derivatives to synthesize arrays on membranes. ... Up to 44 amino acid derivatives can be used, which allows for the use of natural and non-natural amino acids in one run.
|Title||:||Peptide Arrays on Membrane Supports|
|Author||:||Joachim Koch, Michael Mahler|
|Publisher||:||Springer Science & Business Media - 2013-03-09|