Practice of High Performance Liquid Chromatography

Practice of High Performance Liquid Chromatography

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During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on qquantitative analy sisq (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on qpreparative applicationsq (by Wehrli).This may lead to solubility problems and non equilibrium conditions at the head of the separation column when the clear sample is introduced. Those problems result in severe effects on the peak shape. Some examples are given in Figs.

Title:Practice of High Performance Liquid Chromatography
Author:Heinz Engelhardt
Publisher:Springer Science & Business Media - 2012-12-06


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