Protein and Peptide Analysis by LC-MS

Protein and Peptide Analysis by LC-MS

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This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.12.2.4 Preparative Liquid Chromatography Akta Explorer 100 AIR with Fraction Collector, GE Healthcare, Vienna, Austria controlled ... 12.3.1 Mass Spectrometric Detection Select the appropriate mass range to detect your protein envelope. ... The parameters of the ion source and the mass spectrometer should be adjusted according to the instrument manual. For further details and hints see Chapter 2 Parameters such as nebulizer pressure, drying gas flow rates and temperatures areanbsp;...

Title:Protein and Peptide Analysis by LC-MS
Author:Thomas Letzel
Publisher:Royal Society of Chemistry - 2011


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