Protein Purification

Protein Purification

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New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.... Hand homogenizer Mincing (grinding) Moderate Blade homogenizer (Waring type) Grinding with abrasive (e.g., sand, alumina) Vigorous French press Ultrasonication Bead mill Manton-Gaulin homogenizer Lysozyme treatment of bacteria Toluene extraction of ... with glass beads rips cell walls off As for French press above, but on a larger scale It is generally not advisable to use a disruption treatmentanbsp;...

Title:Protein Purification
Author:Robert K. Scopes
Publisher:Springer Science & Business Media - 2013-06-29


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