Recombinant DNA and Biotechnology

Recombinant DNA and Biotechnology

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Laying the foundation; An averview of biotechnology; Genes, genetics, and geneticists; An overview of molecular of molecular biology: recombinant DNA technology; Classroom activities; DNA structure and function; Constructing a paper helix; DNA replication; From genes to proteins;Sizes of the Escherichia coli and human genomes; Extraction of bacterial DNA; Manipulation and analysis of DNA; DNA scissors: introduction to restriction enzymes; DNA goes to the races; Gel electrophoresis of precut lambda DNA; Recombinant paper plasmids; Restriction analysis challenge worksheets; Detection of specific DNA sequences; DNA sequencing; The polymerase chain reaction: paper PCR; Transfer of genetic information; Trasformation of Escherichia coli; Conjugative transfer of antibiotic resistance in Escherichia coli; Transduction of an antibiotic resistance gene; Agrobacterium tumefaciens: nature's plant genetic engineer; Analysing genetic variation; Generating genetic variation: the meiosis game; Analysing genetic variation: DNA typing; A mix-up at the hospital; A paternity case; The case of the bloody knife; The molecularbasis of genetic diseases; Societal issues; Science, Technology, and society; Weighing technology's risks and benefits; Debating the risks of biotechnology; A decision-making model for bioethical issues; BBioethics case study: gene therapy; Bioethics case study: genetic screening; Careers in biotechnology; Appendixes; Laboratory biosafety; Basis microbiological methods; Aseptic technique; Sterilization of equipment and media; Recipes; Biotechnology laboratory equipment; Using the equipment; Recommended reading; Teaching resources; National science education standards and the content of this book; Templates; Overhead masters.Diagram 15.1 Challenge II: possible products with one replication origin and a total size of less than 7, 000 bp. 2, 000 2, 000 ... They must imagine a DNA molecule with a restriction map and generate the data (have them draw a gel!). Let theiranbsp;...

Title:Recombinant DNA and Biotechnology
Author:Helen Kreuzer, Adrianne Massey
Publisher:Amer Society for Microbiology - 1996


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