This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cytoskeletal structure, including such topics as rotational movement of formins studied by fluorescence polarization microscopy, in vitro assembly assays for bacterial actin filaments, and modulators of microtubule plus end growth. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers cytoskeletal structure Contains chapters with such topics as reconstitution of organelle transport and assaying microtubule nucleation by gamma-tubulin ring complex.Engineering Defined Motor Ensembles with DNA Origami Brian S. Goodman and Samara L. Reck-Peterson ... to Motor Proteins Forming the MotoraDNA Origami Complex Single-Molecule Motility Analysis of MotoraDNA Origami Complexes 1. 2. ... Summary and Future Directions Acknowledgments References Construction and Analyses of Elastically Coupled ... and Michael R. Diehl Introduction Synthetic Strategy: A Modular Plug-and-Play Approach Two-Motor Biophysical Assays 1.
|Title||:||Reconstituting the Cytoskeleton|
|Publisher||:||Academic Press - 2014-03-11|