RNA Silencing

RNA Silencing

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RNA Silencing: Methods and Protocols facilitates the translation of gene silencing concepts into practical applications, and includes a broad and useful set of RNA silencing protocols. Sections cover the biochemical aspects of silencing machinery, methods for RNA silencing in nonmammalian organisms, design, preparation, and use of RNAs to silence gene expression, several methos for the in vivo delivery of siRNAs and silencing vectors, and methods for the study and use of microRNAs.Methods The methods presented here describe how to (1) construct the pFastb; HTC-Dicer construct and Dicer bacmid, ... Many of the methods used here require general molecular biology ski] If the instructions provided here or by theanbsp;...

Title:RNA Silencing
Author:Gordon Carmichael
Publisher:Springer Science & Business Media - 2005-01-01


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