Sponges (phylum Porifera) are known to be very rich sources for bioactive compounds, mainly secondary metabolites. Main efforts are devoted to cell- and mariculture of sponges to assure a sustainable exploitation of bioactive compounds from biological starting material. These activities are flanked by improved technologies to cultivate bacteria and fungi which are associated with the sponges. It is the hope that by elucidating the strategies of interaction between microorganisms and their host (sponge), by modern cell and molecular biological methods, a more comprehensive cultivation of the symbiotic organisms will be possible. The next step in the transfer of knowledge to biotechnological applications is the isolation, characterization and structural determination of the bioactive compounds by sophisticated chemical approaches.Approximately 0.3 pmol of each dsDNA target was spotted on a positively charged nylon filter (LI-COR) using a ... kitaquot; reaction mixture (LI-COR) according to the manufacturera#39;s instructions and purified on a GFX column (Amersham/ Pharmacia). ... 5.3.3 Hybridization A filter with the spotted cDNA targets was pre- hybridized in 0.17 ml/cm2 of the Odyssey DNA hybridization solution (LI-COR) at 65 AdC for 1 hanbsp;...
|Author||:||Werner E. G. Müller|
|Publisher||:||Springer Science & Business Media - 2003-01-01|