The results provided by this work support the model that LvsB and Lyst proteins act as negative regulators of fusion by limiting the heterotypic fusion of early with late compartments and antagonize Rab GTPases in membrane fusion. The LvsB localization studies and the functional assessment of the LvsB-null phenotype helped make unique contributions to the understanding of the molecular function of Lyst proteins.Figure 2.1: Knock-in strategy to label LvsB with GFP A) Diagram indicating the construct used to insert the GFP coding region at the 5a#39; ... The endolysosomal compartments of wild type (C), knock-in (D), and LvsB- null (E) cells were labeled byanbsp;...
|Title||:||Study of LvsB in Dictyostelium Discoideum Provides Insights Into the Chediak-Higashi Syndrome|
|Publisher||:||ProQuest - 2007|