Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles. - ;Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes and other organelles. Practical guidance on basic separation techniques needed in all cell biology laboratories is given. These techniques are presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles. -Birkenmeyer and Ray (60) resuspended such pellets in 0.25 M sucrose, 20 mM Tris-HCl pH 7.5 using a manual Teflon-glass homogenizer, and ... The method depends very much on the type of bacterium, whether it is Gram-positive or Gram -negative. ... As an alternative, sonication or the French press (normally two passages at 100-150 MPa) may be used to produce cytoplasmic membrane vesiclesanbsp;...
|Title||:||Subcellular Fractionation : A Practical Approach|
|Author||:||J. M. Graham, D. Rickwood|
|Publisher||:||Oxford University Press, USA - 1997-01-30|