In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.We have used microwave assays to generate chromatograms during protein purification and for general protein determinations ... The modifications we present here are very easily adapted to most commercially available microwave ovens and, in the case of ... Users should consult their equipment manuals and institute safety offices prior to using microwave ovens. ... The positions of these a hota and acolda spots is a function of the physical design of the oven chamber and the electricalanbsp;...
|Title||:||The Protein Protocols Handbook|
|Author||:||John M. Walker|
|Publisher||:||Springer Science & Business Media - 2008-02-12|