Since TRP-ML1 is lysosomally localized and is subject to proteolysis, I wanted to first examine the trafficking and characterize the processing of this protein. TRP-ML1 undergoes cleavage within its first extracellular loop during its biosynthetic delivery to lysosomes. The lysosomal delivery of TRP-ML1 is impaired by depletion of the Adaptor Protein (AP) complex AP-1, while proteolysis remains unaffected in cells lacking AP-1, indicating that cleavage may occur in a pre-lysosomal compartment.Arrows in this diagram represent known or presumed intracellular transport routes. The coat complexes depicted are COPII (blue), COPI (red), and clathrin ( gold). Endocytic carriers and subcellular organelles are labeled as indicated. TGNanbsp;...
|Title||:||The Role of Mucolipin-1 in Pathogenesis of the Lysosomal Storage Disease Mucolipidosis Type IV.|
|Publisher||:||ProQuest - 2008|