NMR relaxation experiments have been used to investigate the role of protein dynamics during catalysis for a hyperthermophilic-mesophilic adenylate kinase pair (Chapter 1). The opening of the nucleotide lids has been determined to be the rate-limiting step for both enzymes, and the difference in these rates account for the lower catalytic activity of the hyperthermophilic enzyme at ambient temperatures.9), three conclusions can be drawn from the results. First ... Labelling sites in the lids were selected on the lusis of distance changes Itiwern the open and closed con for imt inns, consider ingonly ... I pair owing to their high photostability and quantum yield and because of the well-suited Forster radius H, ... Single a#39; molecule measurements were performed with a home built sample-scanning optical confocal microscope, hxcitation light was ... DH-P-635 +ai PDF 80B driver , Picoquant).
|Title||:||The Role of Structure and Dynamics in Adenylate Kinase Catalysis|
|Author||:||Vu Thuong Thai|
|Publisher||:||ProQuest - 2007|