enzymes and related biochemicals Charles C. Worthington ... on supports using Mil- lipore 0.45 micron filter discs which have been wetted with solution A. Each tube is washed with about 3 ml 10% TCA five times. ... X 3.5 (DPM/ 10 nmole substrate) X sample volume X protein concentration of enzyme DNA POLYMERASEa ALPHAa (calf thymus) DNA polymerase a ... 1974b; Chang and Bollum 1972c).
|Title||:||Worthington enzyme manual|
|Author||:||Charles C. Worthington|